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Benesi-Hildebrand (B-H) method was initially put forward by Benesi and Hildebrand in 1949[1] and has become a very popular method for determining equilibrium constant K and stoichiometry of ...
(a) Benesi-Hildebrand plot for determining the binding constant of 1 with F- ion (in THF, pH = 7.0) at 298 K. The binding constant value is 2.55 X 105 M-1. (b) Normalized fluorescence intensity of 1 (3.9 μM) at each concentration of F- (THF, pH = 7.0) at 298 K. A linear curve was obtained from these normalized fluorescence intensity data.
To obtain the binding constant, the Benesi–Hildebrand method was applied for the complex formed between the heavy metals and the CB[5] and CB[7]. To remove dissolved oxygen in solution, the samples were degassed by purging nitrogen gas for more than 10 min prior to the measurement.
Performance of the Benesi-Hildebrand method in cyclodextrin chemistry The relative errors and success rates of the BH method in measuring the binding constants of CD complexation are summarized in Tables 1 and 2, respectively.
This document describes in detail how to determine the binding constants, covering not only the basic prin-ciple but also the practical issue: a theoretical experimental guideline, a representative method for the determination of
The determination of association constants using spectroscopic measurements such as absorbance or fluorescence techniques is commonly accomplished by the method of Benesi-Hildebrand.1-4
Mar 1, 2019 · Abstract Herein, we determined the binding constants for transition metal complexes of Cu2+, Co 2+, Fe 2+, Ni 2+, and Zn 2+ ions formed with the ligand picolinaldehyde-2-pyridylhydrazone (PapyH) using the Benesi-Hildebrand method.
Nov 1, 1997 · The fluorescence intensity remained constant following the addition of greater than 500 μM (excess) Fe3+ and was accompanied by a clear greenish yellow fluorescence. By fitting these data to the Benesi–Hildebrand expression [81,82], a good linear relationship (R = 0.99) was obtained, as shown in the Fig. 6 inset.
By fluorescence method: The binding constant value of Zn2+ and Cd2+ with receptor has been determined from the emission intensity data following the modified Benesi–Hildebrand equation, 1/ = 1/ Imax +(1/Ka[C])(1/ Imax).
Oct 7, 2023 · Benesi–Hildebrand equation can be used to calculate binding constants from the emission data. F0, F, and Fx are the emission intensities, respectively, in the absence of, at intermediate, and infinite concentration (representing saturation level of interaction) of DNA, and Kb is the binding constant.
The Benesi–Hildebrand method is a mathematical approach used in physical chemistry for the determination of the equilibrium constant K and stoichiometry of non-bonding interactions.
The Benesi-Hildebrand method is a mathematical approach used in the determination of the equilibrium constant K and stoichiometry of nonbonding interactions. This method has been typically used to study reaction equilibriums that form 1:1 guest-host complexes, where the guest and host are reactants, as shown below.
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